Journal: Biomedicines

Article Title: Effect of MNCQQ Cells on Migration of Human Dermal Fibroblast in Diabetic Condition

doi: 10.3390/biomedicines10102544

Figure Lengend Snippet: Contents of the QQ Culture Medium.

Article Snippet: rh IL-6 , Peprotec #200-06 , 20 ng/mL.

Techniques: Concentration Assay

Journal: PLoS ONE

Article Title: Mapping the Homodimer Interface of an Optimized, Artificial, Transmembrane Protein Activator of the Human Erythropoietin Receptor

doi: 10.1371/journal.pone.0095593

Figure Lengend Snippet: ( A ) Extracts were prepared from parental BaF3 cells or BaF3/HA-hEPOR cells expressing empty RVY-puro vector (V), EBC5-16, or TC2-3. Where indicated, cells were acutely stimulated with EPO. Samples were immunoprecipitated with anti-HA (3F10) antibody and immunoblotted with anti-phosphotyrosine antibody. Size of protein markers (in kDa) is shown on left. ( B ) Extracts from BaF3/HA-hEPOR cells expressing RVY-puro vector (V), EBC5-16, or TC2-3. Where indicated, cells were acutely stimulated with EPO or RPMI-IL-3 medium. Samples were immunoblotted for phosphorylated JAK2. Blot was reprobed for total JAK2. Size of protein markers (in kDa) is shown on left. ( C ) Extracts from the cells described in ( B ) were immunoblotted for phosphorylated STAT5. Blot was reprobed for total STAT5. Size of protein markers (in kDa) is shown on left.

Article Snippet: For erythroid colony forming assays in methylcellulose, 1×10 4 GFP + -infected cells/mL were washed in Iscove’s Modified Dulbecco’s Medium (L-glutamine, 25 mM HEPES, 3.024 g/L Na 2 CO 3 ) (Gibco) plus 2% FBS (Stem Cell Technologies) and diluted 1∶10 in methylcellulose medium (Methocult H4531, Stem Cell Technologies) containing 20 ng/mL rh-IL-3, 20 ng/mL rh-IL-6 (ConnStem), 50 ng/mL rh-SCF in the presence or absence of 3 U/mL of EPO.

Techniques: Expressing, Plasmid Preparation, Immunoprecipitation

Journal: PLoS ONE

Article Title: Generation of Megakaryocytic Progenitors from Human Embryonic Stem Cells in a Feeder- and Serum-Free Medium

doi: 10.1371/journal.pone.0055530

Figure Lengend Snippet: Differentiating hESCs were cultured for 10 days (d) in BMP4, VEGF, SCF and FGF2 and then for a further 3 or 10 d in TPO, SCF and IL-3. Cells were dissociated at d13 or d20 of culture and stained with antibodies directed against CD41 and a panel of hematopoietic markers. A) Gating strategy identifying CD41 + cells at d13 and B) CD41 lo and CD41 + cells at d20 of hESC differentiation. C) Percent expression of hematopoietic - CD45, CD34, CD43; megakaryocytic – CD110, CD42b, CD61, myeloid – CD33 and hemato/endothelial – CD117 and KDR surface markers on the CD41 expressing cells. (Representative FACS panels stained with isotype controls for CD41 staining are shown in ).

Article Snippet: After 10 days, 72 EBs were transferred to each well of a 6 well flat-bottom tissue culture plate containing 7.2 ml of BPEL supplemented with 20 ng/ml rh thrombopoietin (TPO), 25 ng/ml rhSCF and 25 ng/ml rh interleukin (IL)-3 (Peprotech).

Techniques: Cell Culture, Staining, Expressing